3D Sponge-Matrix Histoculture

This volume describes numerous applications of sponge-matrix histoculture to study cancer biology and the treatment of cancer, stem cells, organoids, growth and repair of nerves, lymphoid tissues that produce antibodies, and HIV infection. The chapters in this book cover topics including, in vivo-like growth patterns of multiple types of tumors in Gelfoam histoculture; development of the histoculture drug response assay (HDRA) for cancer patients; hair-shaft growth in Gelfoam histoculture of skin as well as isolated hair follicles in Gelfoam histoculture; the use of Gelfoam histoculture to discover novel treatment of HIV infection; and imaging DNA repair after UV irradiation damage of cancer cells in Gelfoam histoculture. The volume explains what is true 3D cell and tissue culture and corrects widespread misconceptions of 3D culture in the literature. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cuttingedge and authoritative, 3D Sponge-Matrix Histoculture: Methods and Protocols is a valuable resource that contributes to new cell and tissue culture research, and other diseases based on the true 3D culture.