A Biochemical Study of FOXM1 and TP53 Genes in Breast Cancer Patients

Background: Breast cancer is a malignant tumor arising from the terminal duct lobular unit of the breast tissues. It is most prevalent malignancy in women and its incidence is increasing, approximately equals to 5-10%.Forkhead box M1 (FOXM1) is a transcription factor associates with proliferation signals and over-expressed in majority of human cancer involved breast. TP53 gene negatively regulates FOXM1 expression and therefore inactivation of TP53 in tumors could partially illustrate the phenomenon of FOXM1 over-expression in human cancers. Objectives: The main objective of the current study is to analyze FOXM1 and TP53 gene expressions levels in formalin fixed paraffin embedded tissues of breast cancer specimens.Subjects and Methods: One hundred formalin fixed paraffin embedded (FFPE) tissue samples of malignant breast tumors as well as 100 FFPE samples of normal breast tissues were investigated. Ages of patients (Mean SD) were 48.4 10.90 year with a minimum to maximum of 20 -75 years. Total mRNA was extracted from FFPE blocks by using a specific kit.FOXM1 and TP53 gene expressions were estimated quantitatively by real time PCR.