Axonal Transport

This volume covers a wide range of model systems from invertebrate to humans and in vitroandin vivo. Chapters detail methods on squid, aplysia, xenopus, mouse in vivo, ex vivo, rodent primary neurons, human-derived neurons, zebrafish, drosophila in vivo, primary neurons, c. elegans, and in vitro reconstitution assays.Written in the format of the highly successfulMethods in Molecular Biologyseries, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge,Axonal Transport: Methods and Protocolsaimstofurther our understanding of the trafficking ofcellularcomponents in the nervous system leading to the advancement of basic knowledge that underlines the cell biology of theneuron. The chapters "Live imaging of RNA Transport and Translation in Xenopus Retinal Axons, Retrograde Axonal Transport of Neurotrophins in Basal Forebrain Cholinergic Neurons, Assessment of Mitochondrial Trafficking as A Surrogate of Fast Axonal Transport in Human Induced Pluripotent Stem Cell Derived Spinal Motor Neurons,Drosophila Primary Neuronal Cultures as a Useful Cellular Model to Study and Image Axonal Transport, andIn vitro Reconstitution of Kinesin-Based, Axonal mRNA Transport" are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com