Bacterial Pathogenesis

This volume discusses various methods and protocols used for the experimentation of a wide range of bacterial species, such asStreptococcus pyogenes, Staphylococcus aureus, Streptococcus pneumonia, Listeria monocytogenes,andMycobacterium marinum. Bacterial Pathogens: Methods and Protocolsis divided into 6 parts: Part 1 describes different approaches to identifying and characterizing bacterial effector molecules; Part 2 deals with structural biology of bacterial pathogenesis and how to overcome folding and stability problems with recombinantly expressed proteins; Part 3 details methodology that identifies bacteria in complex communities and how genomes of bacterial pathogens have evolved; Part 4 reflects on the rapid development of advanced imaging techniques that address questions about molecular properties of individual live bacteria, ultrastructure of surfaces, and subcellular localization of bacterial proteins; Part 5 describes methods from in vitro and in vivo modeling of bacterial infections; and Part 6 explores how bacterial pathogens are the true experts of the immune system. Writtenin the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive,Bacterial Pathogens: Methods and Protocolsis a valuable resource for anyone who is interested in this fascinating and evolving field.