Bacterial Secretion Systems

This second edition details new and updated protocols that cover techniques used to study secretion systems. Chapters focus on identifying and localizingthe different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imaging of large complexes, defining the assembly pathway by fluorescence microscopy and the role of energy during assembly and/or secretion, identifying secreted effectors as well as using reporters to follow effector transport.Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge,Bacterial Secretion Systems: Methods and Protocols- Second Editionaimsto be auseful andpractical guide to new researchersand experts looking to expand their knowledge.