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As a part of the systematic and proteomic approach based characterization of plant material with the advantage of producing valuable informations of functional genomics for a general crop improvement programme, leaves of holy medicinal-aromatic plant Tulsi (Ocimum basilicum L.) were used as a source of proteins and a model eukaryotic system. In this study, we developed a reliable protocol for PEG mediated enrichment of low abundant cellular proteins to increase their detection through 2 DE-SDS PAGE. I6% PEG was found to be optimum for the precipitation of Ocimum protein. Further, proteome proteins in different fractions were analysed electrophoretically including 2- DE technique. These protocols may also will helpful in further studies in basil proteomics and other plants also.
- Format: Pocket/Paperback
- ISBN: 9783659235696
- Språk: Engelska
- Antal sidor: 80
- Utgivningsdatum: 2012-09-11
- Förlag: LAP Lambert Academic Publishing