Biocatalysis

Dirk Tischler studied Applied Natural Science with focal points on Environmental Microbiology and Biotechnology as well as Environmental Analytics at TU Bergakademie Freiberg (Germany) and graduated in 2007. He continued in Freiberg with his doctoral studies on styrene monooxygenases and related enzymes and completed his dissertation with distinction in 2012. For this he was awarded with two pre-doctoral scholarships from the Deutsche Bundesstiftung Umwelt and Fulbright and he had two international research stays in Biochemistry Laboratories with Willem van Berkel at Wageningen University and George Gassner at San Francisco State University. The doctoral thesis was awarded with two prizes. He continued as a group leader in the field of industrial biotechnology with emphasis on the prediction of biocatalysts and pathways from (meta)genomes of soil bacteria, especially actinobacteria. This includes three prestigious grants for his group projects GETGEOWEB (ESF), BakSolEx (BMBF), and ChemBioCat (NRW). 2018 he became W1 Professor and was tenured in 2019 to Full Professor for Microbial Biotechnology at the Ruhr University Bochum. He focuses on the identification and application of novel biocatalysts, mostly related to redox biochemistry.

• 1. Biocatalysis: How to select the proper mode of applicationDirk Tischler and Jose Munoz2. Documentation in biocatalysis and data handlingJürgen Pleiss3. Mining metagenomes from extremophiles as resource for novel glycoside hydrolases for industrial applicationsJose Munoz4. Functional MetaproteomicsLars I. Leichert5. Sequence-function relation for the prediction of enzyme properties: A case study on flavin-dependent oxidasesDirk Tischler6. P450 monooxygenase in whole-cell formatCarolin Mügge7. Regio- and Stereospecific oxidation based on di-iron monooxygenases producing whole cellsRolandas Meskys8. Recombinant enzyme expression and targeted mutagenesis in Aromatoleum speciesMaciej Szaleniec9. C.necator as a model organism for CO2-based biotechnologyLars Lauterbach and Sandy Schmidt10. Cupriavidus in whole-cell biocatalysisSandy Schmidt11. Whole-cell biocatalysis with MyxobacteriaMarkus Nett12. Streptomyces for natural product formation: Targeted mutagenesis in PKSFrank Schulz13. Reductive Amination: Methods for cell-free and whole-cell biocatalysisFrancesco G. Mutti, Vasilis Tseliou, Matteo Damian, Josemarco Mendoza-Avila and Marco Rabuffetti14. Challenges and good practices on transaminase-catalysed synthesis of optically pure aminesIoannis V. Pavlidis15. W-enzymes in biocatalysis: Chances and difficulties for the userPeter-Leon Hagedoorn16. Atroposelective biocatalysis employing ADHsGonzalo de Gonzalo17. Applications of alcohol oxidasesJuan Mangas-Sánchez18. Cascades with Oxidoreductases: Enzyme and ChemistryCaroline Emilie Paul19. In vitro biocatalysis with P450 monooxygenasesPimchai Chaiyen20. Construction of artificial fusion proteins for biocatalysisAnna Christina Ngo21. Production and engineering of chimeric UPOsMiguel Alcalde22. Applications of UPOsFrank Hollmann23. Cell free protein synthesis for biocatalysisKatrin Rosenthal24. Immobilization for a stable biocatalyst applicationKatarzyna Szymanska, Karolina Labus, Aleksandra Lambarska and Katarzyna Czyżewska25. Assessment of biocatalytic processesStephan Lütz26. Assay development for PET degradation by biocatalystsÁlvaro Lorente-Arévalo and Aurelio Hidalgo