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Capillary Electrophoresis of Carbohydrates

Inbunden, Engelska, 2003

Av Pierre Thibault, Susumu Honda, Pierre Thibault, Susumu Honda

1 409 kr

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Glycoconjugates such as glycoproteins and glycolipids play important roles in cell-cell interaction events, including development, differentiation, m- phogenesis, fertilization, inflammation, and metastasis. A number of reports have documented the association of unique oligosaccharide sequences to p- tein targeting and folding, and in mechanisms of infection, inflammation, and immunity. For glycoproteins, these glycan appendages are the result of extensive co- or post-translational modifications of the nascent proteins in the endoplasmic reticulum and in the Golgi apparatus. Although nucleic acids and proteins are copied from a template in a repeated series of identical steps using the same enzymes, complex carbohydrates are formed by the sequential actions of cellular glycosyltransferases that specifically recognize unique s- strates. The molecular biology of these transferases and other carbohydra- modifying enzymes is providing important insights on oligosaccharide recognition events.While it is acknowledged that the definition of the protein complement of cells and tissues (the so-called proteome) remains an enormous task in this postgenomic era, the characterization of all glycans produced by individual organisms (referred to as the glycome) presents an equally imp- tant challenge. This task is further complicated by the fact that oligosacc- rides cannot presently be cloned. These complex carbohydrates exist in a staggering diversity of structures, linkages, and branching, thus providing an exquisite molecular repertoire for cellular interactions.

Produktinformation

  • Utgivningsdatum2003-01-14
  • Mått155 x 235 x 19 mm
  • Vikt644 g
  • FormatInbunden
  • SpråkEngelska
  • SerieMethods in Molecular Biology
  • Antal sidor318
  • Upplaga2003
  • FörlagHumana Press Inc.
  • ISBN9780896038264