Cloning, Gene Expression and Protein Purification

This combined lecture and laboratory manual presents detailed protocols for the multi-step process involved in isolating a gene, cloning and characterizing it, expressing the encoded protein, purifying the protein, and characterizing rudimentary aspects of its basic physical properties. The manual includes 20 experiments designed to train students to prepare, manipulate and analyse plasmids, to produce fusion proteins in bacteria, and to purify these proteins based on unique chemical properties or substrate affinities. Maximizing its utility for both students and instructors, this unique manual precedes each lab experiment with the necessary background theory and principles, which culminate in detailed process rationale followed by the actual experimental technique. While many lab manuals list background references, this innovative book combines both theory and procedure into one manageable volume.