Complement regulatory proteins and chronic kidney disease

We examined the expression of the three complement regulatory proteins on erythrocytes, neutrophils, lymphocytes and monocytes from pediatric patients and controls. The proportion of cells expressing the three complement regulatory proteins varied between the cell types we examined, as did the levels of expression of the proteins.Similar to the findings from pediatric patients, there were lower proportion of erythrocytes expressing CD35, CD55 and CD59 in adult patients than in adult controls. In addition, there were lower proportion of CD59-positive neutrophils and CD35-, CD59-positive lymphocytes, and CD59-positive monocytes in adult patients than in adult controls. When looking at protein levels, we observed that the MFI of CD35-positive erythrocytes was significantly higher in adult patients than in the adult control, while CD59-positive neutrophils and monocytes was significantly lower in adult patients than in the adult controls.The findings so far showed that there was, at least to some extent, a correlation between the expression of the complement regulatory proteins and the presence or absence of the disease.