Contributions to the OPT and modeling of amylolysis with REC enzymes

In the present study, the main characteristics and functions of amylase enzymes are summarized. Furthermore the identification and characterization of a new amylase from Photobacterium halotolerans isolated from salt marsh of Tarquinia, Viterbo Italy is described. For a more efficient expression of this enzyme, the Pichia pastoris expression system is studied and optimized with a specific flow rate of methanol during the glycerol-methanol transition phase, using a semiconductor gas sensor for methanol detection from the exhaust air of the fermentor.