Craniofacial Development

This volume explores scientific methodologies currently employed to integrate observational developmental biology, tissue explant and cell-based approaches and genetic/molecular technologies to develop a holistic understanding of craniofacial development. Chapters guide readers through the use of disparate models to study formation of the head and face (c. elegans, zebrafish, mouse, alongside human imaging approaches), together with cell culture, tissue explant andin vivocell imaging and analysis techniques. At the molecular level, chapters include analysing gene expression usingin-situhybridisation andsingle-cell RNA-Sequencing (scRNA-SEQ), as well as genetic modification techniques such asCRISPR/Cas9-mediated deletion.Written in the format of the highly successfulMethods in Molecular Biologyseries, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge,Craniofacial Development: Methods and Protocolsaims to be a guide in the field of craniofacial development for senior and new researchers looking to expand their existing research programs to encompass novel techniques.