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This second volume provides new and updated methods detailing advancements in CRISPR-Cas technical protocols. Chapters guide readers through protocols on prime editing, base editing, multiplex editing, editing in cell-free extract, in silico analysis of gRNA secondary structure and CRISPR-diagnosis. Authoritative and cutting-edge, CRISPR-Cas Methods, Volume 2 aims to serves as a laboratory manual providing scientists with a holistic view of CRISPR-Cas methodologies and its practical application for the editing of crop plants, cell lines, nematode and microorganism. The chapter CRISPR/Cas9-mediated gene editing in human induced pluripotent stem cells is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
- Format: Inbunden
- ISBN: 9781071616567
- Språk: Engelska
- Antal sidor: 397
- Utgivningsdatum: 2021-07-31
- Förlag: Springer-Verlag New York Inc.