Vetenskap & teknik
Directed Molecular Evolution of Proteins
Susanne Brakmann • Kai Johnsson
Inbunden
3139:-
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Natural selection created optimal catalysts. However, optimal performance of enzyme catalysis does not necessarily refer to maximum reaction rate. Rather, it may be a compromise between specificity, rate, stability, and other chemical constraints that makes enzymes capable of catalyzing reactions under mild conditions and with high substrate specificity, accompanied by high regio- and enantioselectivity. The book presented here focuses on the directed evolution of proteins, which has established itself as a powerful method for designing enzymes showing new substrate specificities. It includes a comprehensive repertoire of techniques for producing combinatorial enzyme libraries, while the functional gene expression in a suitable host helps in selecting the appropriate structure, making fast screening a necessity. This book illustrates both the theoretical background as well as the potential of this interesting method in practice - which is becoming ever more important even in classical organic synthesis!
- Illustratör: 15 schwarz-weiße Tabellen 108 schwarz-weiße und 13 farbige Abbildungen
- Format: Inbunden
- ISBN: 9783527304233
- Språk: Engelska
- Antal sidor: 368
- Utgivningsdatum: 2002-03-01
- Förlag: Blackwell Verlag GmbH