Evaluation and validation of rapid molecular methods for the detection and identification of microorganisms in water - Standard Operating Procedures 1

bokomslag Evaluation and validation of rapid molecular methods for the detection and identification of microorganisms in water - Standard Operating Procedures

Vetenskap & teknik

Evaluation and validation of rapid molecular methods for the detection and identification of microorganisms in water - Standard Operating Procedures

Paul Monis • Nic Reid • Australian Water Quality Centre • South Australian Water Corporation

Pocket

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36 sidor
2015

Molecular techniques are emerging as a rapid and powerful approach for microorganism detection. This project reviewed the efficacy of PCR assays for Cryptosporidium, Microcystis, adenovirus and ammonia oxidising bacteria, as well as candidate techniques for DNA extraction and inhibitor removal. The literature review led to an evaluation of DNA extraction kits and reagents for PCR; finalisation of assay formats; the development of PCR controls and Standard Operating Procedures (SOP); analysis of assay robustness using real samples; and completion of an Inter-laboratory trial using the SOPs developed. This book is co-published with Water Research Australia.

Författare: Paul Monis, Nic Reid, Australian Water Quality Centre, South Australian Water Corporation
Format: Pocket/Paperback
ISBN: 9781780407517
Språk: Engelska
Antal sidor: 36
Utgivningsdatum: 2015-06-15
Förlag: IWA Publishing