Fluorescent Proteins

This volume brings together cutting-edge laboratory protocols to characterize the novel fluorescent proteins (FPs) and approaches based on fluorescent proteins that aim to answer some of the key cell biological questions. The book covers topics ranging from the database of fluorescent proteins to their characterization and adaptationto a wide rangeofbiological systems. Written for the highly successfulMethods in Molecular Biologyseries, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical,Fluorescent Proteins: Methods and Protocolsserves as an ideal guide for students and academicians enthusiastic about the recent progress in the practical application of fluorescent protein technology.