Functional Genomics in Medicago truncatula

This volume discusses popular methods to achieve different types of mutagenesis and forward/reverse genetics inMedicago truncatula.Several studies on genetic control of developmental and metabolic processes in this model legume are also described. The chapters in this book cover topics such as Targeting Induced Local Lesions IN Genomes (TILLING),Fast Neutron Bombardment (FNB),Tnt1insertional mutagenesis,Virus-Induced Gene Silencing (VIGS),stable inactivation of microRNAs in roots, gene editing by CRISPR-Cas9, etc.This book also contains reviews on the specific use of these techniques in functional studies on thegenetic control of seed, leaf, root, nodule, floral and fruit development inM. truncatula.Written for the highly successfulMethods in Molecular Biologyseries format, chapters contain the kind of detailed description and implementation advice needed to promote success in the lab. Cutting-edge and thorough,Functional Genomics in Medicago truncatula: Methods and Protocolsis a valuable resource for anyone interested in learning more about this developing field.