Handbook of Flow Cytometry Methods

Handbook of Flow Cytometry Methods Edited By J. Paul RobinsonAssociate Editors: Zbigniew Darzynkiewicz, Phillip Dean, LynnDressler, Hans Tanke and Leon Wheeless Here is the ideal laboratorymanual for investigators involved in research and diagnosticapplications of flow cytometry. Handbook of flow cytometry methodsoffers a collection of proven, basic procedures designed for use inthe flow cytometry laboratory. Methods implemented at PurdueUniversity Cytometry Laboratories and developed in laboratories ofcontributors worldwide provide practical, step-by-step proceduresfor: * the preparation and handling of standards,reagents andspecimens * laboratory analyses for immunophenotyping * measurement of DNA content and cell cycle analyses * determination of cell physiology and function * set up and operation of flow cytometers * computational and data analysis techniques * safety precautions Drawing on their collective experience at leading research centers,the editors have fashioned a unique "working manual" intended toserve as the starting point for the evaluation and establishment oflaboratory benchmark standards in flow and image cytometry. Eachmethod is described in detail to be used and modified depending onthe user's needs. Special features of Handbook of Flow CytometryMethods include: * ample space throughout for personal annotations * suggestions of alternative procedures; trouble-shootingtips * extensive list of reagents and suppliers * comb-bound format for ease of use at the lab bench Handbook of Flow Cytometry Methods is an essential laboratoryresource for students, faculty, and researchers in cell andmolecular biology, immunology, cancer biology, hematology/oncology,and laboratory medicine.