Histone Methyltransferases

This volume provides methods used to investigate histone methyltransferase function. Chapters guide readers throughacomprehensive set of approaches that detailphylogenetic diversity, histone demethylase activitiesin vitro,generating chromatin substrates, auto-methylation, quantification of metabolites, protein purification, crystallization, X-ray structure, cryogenic electron microscopy,assessinggenome-wide patterns, CUT&Tag in mouse embryonic tissues, chemical biology approaches, peptide SPOT arrays, nascent chromatin capture, ectopic protein tethering, computational models, anddevelopment of methyltransferase inhibitors.Written in the format of the highly successfulMethods in Molecular Biologyseries, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge,Histone Methyltransferases: Methods and Protocolsaimsto be auseful and practical guide to new researchersand experts looking to expand their knowledge.