HIV Reservoirs

This book details the development of methods and models to study the HIV-1 viral reservoir with the ultimate goal of achieving a functional cure of HIV infection. Chapters are divided into six parts coveringcell lines,in vitroand ex vivo primary cell models of persistent infection, in vitroand ex vivo tissue-derived models, infected animal models human immune cells, methods of detection and analysis of the reservoir, and current approaches to achieve either a functional cure or cART-free long-term remission.Written in the format of the highly successfulMethods in Molecular Biologyseries, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge,HIV Reservoirs: Methods and Protocolsprovidesa comprehensive, updated collection of state-of-art methodologies and models to tackle the HIV-1 viral reservoir.