Methods in Plant Biotechnology

The book presents various essential techniques of recombinant DNA technology, such as the isolation and transformation of bacteria, the isolation and characterization of nucleic acids, the construction and screening of gene libraries, DNA sequencing, RFLP and RAPD gene mapping, genetic transformation, and molecular analysis of transgenic plants. Additionally, the book provides protocols for important techniques currently used in plant biotechnology. It also includes methods for RFPL and RAPD analysis, as well as a practical example of an actual research problem in the field of plant genetic engineering. The text is complemented by a glossary of important terms and the appendix provides information on the preparation of common buffers and reagents used in the various protocols.