Molecular Approaches for the Detection of Fungal Phytopathogens

The book discusses recent advancement and updates in the molecular diagnostics of fungal phytopathogens. The techniques covered in the seventeen chapters helps in understanding the importance of early detection of fungal pathogens and their toxins in both post and pre-harvest stages of crop production. The chapters describe the fungal identification and isolation from different sources such as food, feed and cereals using molecular approaches like variants of Polymerase Chain Reaction (PCR) involving generic (genus and species) and gene specific primers, RNA-sequence-based next generation sequence, microarray, Serial Analysis of Gene Expression, Fluorescence In Situ Hybridization, Loop-mediated isothermal amplification, Macroarray, PCR-Denaturing Gradient Gel Electrophoresis, Magnetic Capture Hybridization-PCR, nanotechnology, immunoassays providing faster and sensitive approach than traditional methods that overcome the challenges by developing novel disease control strategies. Features Explores various diagnostic strategies for phytopathogens. Discusses early detection methods when fungal spores are low or no visible symptoms. Provides a historical perspective and compares the traditional methods of detection with the current molecular techniques. Describes details protocol of every techniques described. Covers point-of-care testing for fungal pathogens and quality assurance for molecular testing in plant health.