PCR Primer Design

This third edition provides new and updated chapters on design PCR primers for successful DNA amplification. Chapters are divided into seven parts, including primer design strategies for quantitative PCR, genotyping, multiplex PCR,in silicoPCR primer design, and primer design to identify plant and animal viruses. Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible,PCR Primer Design, Third Editionaims to be useful forvarious fields of molecular biology, including biotechnology, molecular genetics, and recombinant DNA technology.