bokomslag Protein-ligand Interactions
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Protein-ligand Interactions

G Ulrich Nienhaus

Inbunden

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  • 525 sidor
  • 2005
A readily reproducible collection of established and emerging techniques for studying the interaction between proteins and ligands, including biochemical/bulk techniques, structure analysis, spectroscopy, single-molecule studies, and theoretical/computational tools. Among the highlights are surface plasmon resonance (SPR) and reflectometric biosensor approaches, high-throughput screening with confocal optics microscopy, single molecule fluorescence and fluorescence correlation spectroscopy (FCS), atomic force microscopy (AFM), crystallography of reaction intermediates, and time-resolved x-ray crystallography. The protocols follow the successful Methods in Molecular Biology(TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
  • Författare: G Ulrich Nienhaus
  • Format: Inbunden
  • ISBN: 9781592599127
  • Språk: Engelska
  • Antal sidor: 525
  • Utgivningsdatum: 2005-04-01
  • Förlag: Humana P.,U.S.