Protein NMR Techniques

This second edition of Protein NMR Techniques is well written with a dynamic approach that covers multiple topics within its nineteen chapters. The text opens with a review of recombinant protein expression using two organisms, E. coll and P. pastoris that can produce high yields of isotopically labeled protein at a reasonable cost. The focus then shifts slightly to studies of aligned molecules, starting with a chapter on different options for the preparation of an aligned sample. The text also provides a comprehensive review of the use of RDCs to the study protein dynamics highlights and the range of information accessible using these methods. The book concludes with a concise explanation of the application of solid-state methods and the study of membrane proteins, a particularly important but difficult class of targets. Protein NMR Techniques, Second Edition will prove an invaluable resource to biochemists and researchers alike.