Quantitative Gas Chromatography-Mass Spectrometry

Quantitative Gas Chromatography-Mass Spectrometry: Principles in Life Sciences and Biochemical Applications outlines the main principles of Gas Chromatography-Mass Spectrometry (GC-MS), and the ways in which this technique can be applied in fundamental research and applied Life Sciences. The book begins with detailed descriptions of the fundamentals underpinning gas chromatography-mass spectrometry, gas chromatography-tandem mass spectrometry and principles of liquid chromatography-tandem mass spectrometry with a focus upon stable isotopes, labelled analogs, as internal standards within all three techniques. The later chapters then build on this foundation, to serve as a comprehensive guide for method development and validation and quality control in a number of experimental settings. In addition to assisting readers in selecting a pathway, the book provides details and examples of common issues and errors related to GC-MS and helps the reader in helping the readers in troubleshooting and solving these analytical problems. Quantitative Gas Chromatography-Mass Spectrometry then builds on these basic principles to progress onto more complex topics and advanced techniques with a discussion of various applications in industry and high-level academic study research. This book will appeal primarily to researchers, academics and graduate students working with analytical chemistry techniques such as gas chromatography and mass spectrometry. This book will introduce the fundamentals of these techniques and build upon this theoretical foundation to expand into practical applications. This combination of fundamentals and applications to determine target analytes in biological samples is a comprehensive approach that will appeal to researchers in fields such as Biochemistry, Analytical Biochemistry, Mass Spectrometry, Pharmacology, Forensic Medicine, Metabolomics, Life Sciences, Paediatrics and Laboratory Medicine.

• Features structured a learning pathway, starting with basic principles and progressing to more complex topics, ensuring that readers can build a strong foundation before moving on to more advanced techniques
• Delves into advances and applications in the analysis of biological samples, with uses in fields such as Biochemistry, Analytical Biochemistry, Mass Spectrometry, Pharmacology, Forensic Medicine, Metabolomics, Life Sciences, Paediatrics and Laboratory Medicine
• Addresses the whole analytical process comprehensibly from a practical point of view, describing potential analytical challenges, and provides possible solutions to overcome shortcomings and to avoid pitfalls
• Outlines the utilization of stable isotopes (labelled analogs) as internal standards in quantitative analyses, and the way this can be applied to the results of quantitative tandem gas chromatography-mass spectrometry