Techniques and Protocols in Molecular Biology

Nyhet

Häftad, Engelska, 2025

Av Buddhi Prakash Jain, India) Prakash Jain, Buddhi, Ph.D. (Assistant Professor, Department of Zoology, School of Life Sciences, Mahatma Ghandi Central University, Motihari, Bihar

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Techniques and Protocols in Molecular Biology delivers detailed practical guidance for a variety of molecular techniques and procedures. Each chapter focuses on a different technique including isolation techniques for DNA and RNA, gene cloning, PCR, ELISA, and cell culture. These protocols include a detailed description of the technique along with the main principles, required equipment, and comprehensive steps to assist researchers with replicating the protocol and performing the techniques themselves.
This foundational reference is essential for students and researchers looking to enhance their understanding of key molecular techniques and reproduce them successfully in their own laboratory work.

Provides step-by-step instructions to guide researchers in carrying out a range of molecular biology techniques
Includes details of instrumentation, reagents, and buffers necessary for each experiment
Features diagrams to help illustrate the procedures and techniques covered

Produktinformation

Utgivningsdatum2025-10-28
Mått191 x 235 x 10 mm
Vikt450 g
FormatHäftad
SpråkEngelska
Antal sidor222
FörlagElsevier Science
ISBN9780443141607

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Biologi inom Naturvetenskap och teknik

Dr. Buddhi Prakash Jain is an Assistant Professor at the Department of Zoology, Mahatma Gandhi Central University, Motihari, Bihar, India. He completed his doctoral studies at the School of Life Sciences, Jawaharlal Nehru University (JNU) New Delhi, India. His research interests include gene expression regulation and endoplasmic reticulum stress in cancer. He is the recipient of several awards including a gold medal in Master Programme (M.Sc.), EMBO travel award, DST (Department of Science and Technology) Govt of India, Young Scientist travel award, and has published several papers in reputed international journals and a number of books and book chapters.

1. Genomic DNA isolation (Bacteria, plant, and animal cells/tissues)2. RNA isolation from mammalian cells/tissues, Drosophila, and Caenorhabditis elegans using monophasic lysis reagent3. Reverse-transcription polymerase chain reaction4. Quantitative reverse-transcription polymerase chain reaction: Real-time polymerase chain reaction5. Agarose gel electrophoresis6. 3’RACE (random amplification of cDNA end7. 5’RACE (Random amplification of cDNA end8. Plasmid isolation and purification (Alkaline lysis method)9. Preparation of competent cells (Bacterial cells) and transformation (Hanahan’s method)10. Molecular gene cloning11. End-labeling of DNA (5’end labeling by T4 polynucleotide kinase)12. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis and native polyacrylamide gel electrophoresis13. Western blotting14. Electrophoretic mobility shift assay for DNA-protein interaction15. Silver staining of proteins in acrylamide gel compatible with mass spectrometry: Acidic method (From Shevchenko et al., 1996)16. Animal cell culture (Adherent cells)17. Cell viability by MTT assay18. Lactate dehydrogenase assay19. Luciferase assay20. Co-immunoprecipitation21. Protein purification (His-tagged proteins by immobilized metal affinity chromatography)22. Subcellular fractionation by differential centrifugation (Separating nuclear, membrane, and cytosolic fraction)23. DNase 1 footprinting24. Chromatin immunoprecipitation-qPCR25. Flow cytometry for cell surface markers26. Basics of bioinformatics27. GST pull down assay28. Fluorescence In Situ hybridization from paraffin-embedded tissues29. An overview of yeast two-hybrid assay30. Enzyme-linked immunosorbent assay