The Restructurization of the Chromatin of Neutrophilic Granulocytes in Norm and Pathology

There were studied the characteristics of spontaneous and induced chromatin restructuring of neutrophilic granulocytes of peripheral blood in healthy people and patients with various diseases. It was found that the levels of the restructurization of nucleus chromatin of neutrophilic granulocytes were differ in healthy people and in patients with different variants of pathologies. The levels of the restructurization of nucleus chromatin of neutrophilic granulocytes were associated with the nature and severity of the disease process. So in duodenal ulcer complicated by stenosis, there was a pronounced of the restructurization of nucleus chromatin of neutrophilic granulocytes. In patients with rheumatoid arthritis the levels of the restructurization of the nucleus chromatin of neutrophilic granulocytes indicated significant hyperactivation of neutrophilic granulocytes of peripheral blood and of synovial fluid. It was also found that the neutrophilic granulocytes of purulent discharge in patients with the acute phlegmon of the maxillofacial region were exhibited the high levels of the restructurization of nucleus chromatin, which were decreased in according with the positive changing of reparative processes in the wound. In acute destructive pancreatitis and in leptospirosis the levels of the restructurization of chromatin of neutrophilic granulocytes were also very high, but in the cases of the very severe acute destructive pancreatitis and leptospirosis the restructurization of the chromatin of neutrophilic granulocytes were decreased. In neoplastic diseases such as colorectal cancer and were the contrary in respect to each other. So in chronic myelogenous leukemia the levels of the restructurization of nucleus chromatin of neutrophilic granulocytes were very high and in colorectal cancer it was observed decreasing neutrophilic granulocytes and its total blockage of response after induction.

Thus, developed new approaches to the assessment of the functioning of the neutrophilic granulocytes using the new methods of testing the restructurization of nucleus chromatin of neutrophilic granulocytes in healthy people and in patients with different variants of pathologies. These data can be used in the future as diagnostic and prognostic criteria in the assessment of the functioning of the neutrophilic granulocytes in healthy individuals and different diseases.