Yeast Metabolic Engineering

This second edition volume expands on the previous edition with new and updated chapters on the latest developments in the study of yeast within the biotechnology field. The chapters in this book cover topics such as transformation protocols for genetic engineering ofSaccaromyces cerevisiaeandKomagataellaspp.; an overview of selection markers, promoters, and strains used for metabolic engineering ofS. cerevisiae, P. pastoris,andZ. bailii; the use of yeast in CRISPR/Cas9 technology; tools to study metabolic pathway inYarrowia lypolitica; and a discussion on the "universal expression system" that is applied in a broad spectrum of fungal species. Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, andtips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Yeast Metabolic Engineering: Methods and Protocols, Second Editionis a valuable resource for researchers and scientists interested in learning more about this important and developing field.