Brown Adipose Tissue

Methods and Protocols

Häftad, Engelska, 2022

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This detailed volume explores techniques for researching brown adipose tissue (BAT) and the fascinating biology and therapeutic potential of thermogenic adipocytes.

Produktinformation

Utgivningsdatum2022-02-16
Mått178 x 254 x 18 mm
Vikt619 g
FormatHäftad
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor309
FörlagSpringer-Verlag New York Inc.
ISBN9781071620892

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Kemi inom Naturvetenskap och teknik
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Brown Adipose Tissue: A Short Historical Perspective.- Brown Fat Anatomy in Humans and Rodents.- Analysis of Thermogenesis Experiments with CalR.- A Clearing Method for Three-Dimensional Imaging of Adipose Tissue.- Activating Human Adipose Tissue with the β3-Adrenergic Agonist Mirabegron.- Fluorescent and Luminescent Methods to Detect Lipolysis.- Metabolic Turnover Studies to Quantify Energy Uptake by Thermogenic Adipose Tissues of Mice.- Stable Isotope Tracing and Metabolomics to Study In Vivo Brown Adipose Tissue Metabolic Fluxes.- Activation of UCP1-Independent Ca2+ Cycling Thermogenesis by Wireless Optogenetics.- Measurement of Futile Creatine Cycling Using Respirometry.- Analysis of Enhancers and Transcriptional Networks in Thermogenic Adipocytes.- Diet-Induced Thermogenesis: Principles and Pitfalls.- Fluorescent Genetic Tools for Studying Brown Fat Development and Function in Mice.- Isolation and Characterization of Human Brown Adipocytes.- Setting Ambient Temperature Conditions to Optimize Translation of Molecular Work from the Mouse to Human: The ‘Goldilocks Solution’.- Signaling Lipidomic Analysis of Thermogenic Adipocytes.- Blood Sampling for Arteriovenous Difference Measurements Across Interscapular Brown Adipose Tissue in Rat.- In Vivo Imaging of Brown Adipose Tissue in Humans: FDG-PET/CT and Beyond.- Analysis of Single Cell/Nucleus Transcriptome Data in Adipose Tissue.