Histochemistry

Jinsong Zhou, Xi’an Jiaotong University, Shannxi Province, China

• ContentsChapter 1 Introduction1.1 The contents and theories in Histochemistry1.2 The basic requirements of Histochemistry methods Chapter 2 The Tissue Preparation2.1 Tissue collection2.2 Fixation2.3 Tissue washing-up, dehydration and clearance2.4 Embedding2.5 Sectioning2.6 Adherence and mounting2.7 Buffer Chapter 3 The Carbohydrate and its Derivatives Histochemistry3.1 Classification3.2 Histochemistry methods Chapter 4 The Nucleic Acid Histochemistry4.1 DNA histochemical demonstration4.2 Comparison demonstration of DNA and RNA Histochmistry Chapter 5 The Lipid Histochemistry5.1 Overview5.2 Demonstration of lipids with physical methods5.3 Demonstration of lipids with chemical methods Chapter 6 Enzyme Histochemistry6.1 Enzyme and its basic histochemical theory6.2 Histochemistry for common enzymes Chapter 7 The Basic Theory of Immunohistochemistry7.1 Basic Immunology7.2 Common markers and their detection7.3 Basic conditions Chapter 8 Common Methods Used in Immunohistochemistry8.1 Principles8.2 Immunofluorescence method8.3 Immunoenzyme technique8.4 Avidin-biotin method8.5 Protein A method8.6 Immunogold method and immune gold-silver method Chapter 9 Specificity and Sensitivity of Immunohistochemistry9.1 Specificity and specific staining9.2 Control experiment9.3 The methods to enhance immunohistochemical sensitivity Chapter 10 The Double-Staining in Immunohistochemistry10.1 Double-staining Immunohistochemistry on serial section10.2 Immunofluorescence double labelling technique10.3 Immunoenzyme double staining technique10.4 Immunoenzyme-Immunofluorescence double staining10.5 Immunoenzyme-Immunogold double staining Chapter 11 The Lectin Histochemistry11.1 Characteristics and application of lectin11.2 Application of lectin Histochemistry Chapter 12 The progress of in Situ Display12.1 The envision method12.2 The catalysed signal amplification method12.3 The ferric oxide alternative method for HRP Chapter 13 In Situ Hybridization Immunohistochemistry13.1 Basic principle13.2 Probe preparation13.3 Procedure of in situ hybridization histochemistry13.4 Factors affecting in situ hybridization13.5 Control test Chapter 14 In Situ Polymerase Chain Reaction Technique14.1 Basic principle14.2 Basic types14.3 Procedure14.4 Application of in situ PCR technology Chapter 15 Electron Microscopic Cytochemical Technique15.1 Electron microscopic enzyme cytochemistry technology15.2 Electron microscopic Immunocytochemical technique Chapter 16 The Quantitative Assay of Histochemistry Results16.1 The photomicrography16.2 The image analyzer16.3 The flow cytometry16.4 The laser scanning confocal microscopy References