Micropatterning in Cell Biology, Part A

Matthieu Piel and his team develop microfabricated and microfluidic tools to quantitatively control the physical parameters of the cell’s environment and study how cells grow, divide and migrate. The team focused on how physical confinement, geometry and forces affect cell division and cell migration. The general aim of these studies is to draw a line between the physics of the active matter cells are made of and the behavior of cells in the complex environment of tissues, in the context of the immune response and tumor development.

• Section 1: Micro-patterning in 2DCh1 Stamp-off” to micropattern sparse, multicomponent featuresCh2 Poly(vinyl alcohol)-Micropatterned Surfaces for Manipulation of Mesenchymal Stem Cell FunctionsCh3 Patterning of polymeric cell culture substratesCh4 Photopatterning with a Printed Transparency Mask and a Protein-Friendly PhotoresistCh5 Plasma Micro-Contact Patterning : a technique for the precise control of surface patterning at small-scaleCh6 Patterning GradientsSection 2: Maskless MicropatterningCh7 Laser-assisted absorption by photobleaching Ch8 Micropatterning with a liquid-crystal display (LCD) projectorCh9 Cell patterning by Laser-assisted bioprintingCh10 Stereomask lithography for multi-protein patterningCh11 Fabrication of Multifaceted, Micropatterned Surfaces and Image-Guided Patterning Using Laser Scanning LithographyCh12 Print-to-print: Printer-enabled Out-of-cleanroom Multi-object Microprinting MethodSection 3: 2D NanopatterningCh13 Soft/Elastic Nano-Patterned Biointerfaces in the Service of Cell BiologyCh14 Combinatorial Screening of Mesenchymal Stem Cell Adhesion and Differentiation Using Polymer Pen LithographyCh15 High fidelity nanopatterning of proteins onto well-defined surfaces through subtractive contact printingCh16 The use of microarrays and fluorescence in situ hybridisation for the study of mechanotransduction from topography