Molecular Biology and Biotechnology

Microbial Methods

Häftad, Engelska, 2010

Av Manoj V. Parakhia, Rukam S. Toma, Sunil V. Patel, B.A.Golakiya

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Although microbes are small in size, they possess intricate structures at the molecular level. The book Molecular Biology and Biotechnology provides an extensive range of laboratory protocols and practical techniques that are essential for both research and teaching purposes. This text is divided into 370 pages, comprising of 36 chapters, followed by a detailed glossary. It serves as a valuable resource for microbiology researchers, teachers, and students alike.

Produktinformation

Utgivningsdatum2010-01-15
Mått150 x 840 x 15 mm
Vikt840 g
FormatHäftad
SpråkEngelska
Antal sidor382
FörlagNew India Publishing Agency
ISBN9788119235179

Tillhör följande kategorier

Biokemisk teknik inom Naturvetenskap och teknik
Biologi inom Naturvetenskap och teknik

Manoj V. Parakhia: Department of Biochemistry, Junagarh Agricultural University, Junagarh 362 001, Gujarat, IndiaRukam S. Tomar: Department of Biochemistry, Junagarh Agricultural University, Junagarh 362 001, Gujarat, IndiaSunil V Patel: Department of Biochemistry, Junagarh Agricultural University, Junagarh 362 001, Gujarat, India.B.A. Golakiya: Department of Biochemistry, Junagarh Agricultural University, Junagarh 362 001, Gujarat, India.

1. Laboratory safety rules. 2. Milestones in DNA history and biotechnology. 3. Contribution in development of DNA and biotechnology. 4. Equipments required in a molecular marker laboratory. 5. Basic calculations used in microbial biotechnology. 6. Basic principals in preparation of reagents required in microbial biotechnology. 7. Isolation of bacterial genomic DNA. 8. Isolation of RNA from yeast. 9. Nucleotide composition of RNA. 10. DNA melting point determination. 11. Quantitative determination of DNA by DPA method and spectroscopic method. 12. Estimation of RNA by Orcinol method. 13. Electrophoresis. 14. SDS-PAGE electrophoresis. 15. Polymerase chain reaction. 16. Restriction enzyme digestion. 17. Extraction of DNA fragments from Agarose Gel. 18. Isolation the Mammalian DNA from whole blood. 19. Isolation of Plasmid DNA from bacteria. 20. Random Amplified Polymorphic DNA (RAPD). 21. Amplified Fragment Leanth Polymorphism (AFLP). 22. Southern blotting. 23. Ligation of insert DNA to Vector DNA. 24. Bacterial transformation. 25. Detection of plasmid by transformation. 26. Conjugation in E.Coli by plate method. 27. Isolation of Lactose non fermentor mutants of E.Coli by Physical mutagenesis. 28. Isolation of auxotrophic mutant by chemical (5 Bu) mutagenesis. 29. Mutagenesis (direct selection). 30. Mutagenesis (indirect selection). 31. Agrobacterium mediated gene transfer from bacteria to plant. 32. Western blotting. 33. Northern blotting. 34. PCR-sequencing. 35. Overview of metagenomics. 36. Calculation of similarity index values and construction of Dendrogram using NTSYSpc 2.0.