Plant Secondary Metabolism Engineering

Methods and Protocols

Inbunden, Engelska, 2022

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This second edition provides detailed practical information on important methods employed in the engineering of plant secondary metabolism pathways.

Produktinformation

Utgivningsdatum2022-05-05
Mått178 x 254 x 21 mm
Vikt722 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor262
Upplaga2
FörlagSpringer-Verlag New York Inc.
ISBN9781071621844

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Extraction and Analysis of Phenolic Compounds From Grape Berries.- Construction of the UDP-glucose biosynthetic Enzyme Gene Co-Expression Plasmid For Prunasin Production in Escherichia coli.- Histochemical Localization of Phenolic Compounds And Reactive Oxygen Species In Eucalypt Microcuttings.- A Bioinformatics Tool for Efficient Retrieval of High-Confidence Terpene Synthases (TPS) and Application to the Identification of TPS in Coffea and Quillaja.- Use of Catharanthus roseus Cell Cultures for the Synthesis Of Metal Nanoparticles.- Fungal Pigment Assisted Silver Nanoparticle Synthesis and their Antimicrobial and Cytotoxic Potential.- Nuclei Enrichment from Sugarcane Stems for Proteomics Analyses.- Visualization of Suberization and Lignification in Sugarcane.- Isolation of Promoters and Transcription Factors Involved In The Regulation of Lignin Biosynthesis in Saccharum species.- Soapbark Triterpenes: Quillaja brasiliensis Cell Culture Sapogenin and Free Sterol Analysis by GCMS.- Generation of stable Catharanthus roseus hairy root lines with Agrobacterium rhizogenes.- Analyzing the Distribution of Specialized Metabolites From Plant Native Tissues With Laser Desorption Low-Temperature Plasma Mass Spectrometry Imaging.- Virus-Induced Gene Silencing as a Tool to Study Regulation of Alkaloid Biosynthesis in Medicinal Plants.- Cyclotide isolation from Psychotria brachyceras and Psychotria leiocarpa.- Formulation of IMXQB: nanoparticles based on Quillaja brasiliensis saponins to be used as vaccine adjuvants.- Isolation of specialized plant cells by fluorescence-activated cell sorting.- X-Ray Computed Tomography for 3D Anatomical Analysis of Resin Ducts.- Untargeted metabolomic analysis of non-volatile and volatile glucosinolates in Brassicaceae.- Methods of mimosine extraction fromLeucaena leucocephala (Lam.) de Wit leaves.- Microbial production, extraction, and quantitative analysis of isoprenoids.